The modified SDS-alkaline lysis was used to lyse the cells, the new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt.,
Product Introduction
introduction
Code No
Reagent
Price
NG223S
10T
Request a quote
NG223M
100T
Request a quote
Introduction:
The modified SDS-alkaline lysis was used to lyse the cells, the new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt., and then the impurities and other bacterial components were removed by rinsing solution, finally, the purified plasmid DNA was eluted from the silica matrix membrane by low salt and high pH elution buffer. Product Specifications:
The kit is quick and easy.
0.2-1.5 mg of pure high-copy plasmid DNA could be rapidly extracted from 150-200 ml Escherichia coli.
The unique technology of kit can remove endotoxin effective and the transfection effect is excellent.
Unique proprietary formula removes endotoxins, resulting in extremely low endotoxin content (<0.1 EU/μg DNA) and excellent cell transfection.
This product uses a unique solution system that can process various plasmid vectors (including high, medium, and low copy numbers, and large plasmids such as BACs, cosmids, etc.), and can also be directly used for various molecular biology experiments such as restriction enzyme digestion, transformation, PCR, in vitro transcription, and sequencing.