Hligene Yeast mini plasmid purification kit-Hlingene

Product Introduction

introduction

Code No	Reagent	Price
NG207S	100T	Request a quote
NG207M	200T	Request a quote

Reagents (provided by yourself);
Sorbitol buffer: 1.2 M sorbitol prepared with 0.1 M sodium phosphate buffer (pH 7.4): 77.4 ml of 0.1 M sodium phosphate buffer (pH 7.4) and 22.6 ml of 0.1 mol/L NaH2PO4; take 30 ml of sorbitol buffer to dissolve lysozyme in the 100-test kit, and so on, or add as needed for each experiment.
Introduction：
The modified SDS-alkaline lysis was used to lyse the cells, the new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt., and then the impurities and other bacterial components were removed by rinsing solution, finally, the purified plasmid DNA was eluted from the silica matrix membrane by low salt and high pH elution buffer.

Download: Manual (PDF)