In the presence of highly disordered salts, DNA fragments are adsorbed on a silica matrix membrane in a centrifuge column, followed by a series of rapid rinsing-centrifugation steps,
Product Introduction
introduction
Code No
Reagent
Price
NG211S
100T
Request a quote
NG211M
200T
Request a quote
Introduction:
The modified SDS-alkaline lysis was used to lyse the cells, the new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt., and then the impurities and other bacterial components were removed by rinsing solution, finally, the purified plasmid DNA was eluted from the silica matrix membrane by low salt and high pH elution buffer. Product Specifications:
The adsorption amount among the columns is very small, and the repeatability is good.
PE can effectively remove the residual nuclease, even the nuclease-rich strains such as JM series, HB101 can be easily removed.
The kit is fast and convenient, without the use of toxic phenol, chloroform and other reagents, nor the need for ethanol precipitation. The obtained plasmid has high yield and purity which can be directly used in molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing and so on.