The modified SDS-alkaline lysis was used to lyse the cells, the new lysis buffer allows the adsorption o-f DNA onto silica membrane in the presence of high salt.,
Product Introduction
introduction
Code No
Reagent
Price
NG201S
100T
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NG201M
200T
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Introduction:
The modified SDS-alkaline lysis was used to lyse the cells, the new lysis buffer allows the adsorption o-f DNA onto silica membrane in the presence of high salt., and then the impurities and other bacterial c-omponents were removed by rinsing solution, finally, the purified plasmid DNA was eluted from the silica matrix membrane by low salt and high pH elution buffer. Product Specifications:
The adsorption amount among the columns is very small, and the repeatability is good.
PE can effectively remove the residual nuclease, even the nuclease-rich strains such as JM series, HB101 can be easily removed.
The kit is fast and convenient, without the use of toxic phenol, chloroform and other reagents, nor the need for ethanol precipitation. The obtained plasmid has high yield and purity which can be directly used in molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing and so on.