4008760968
Tissue and Cell RNA Extraction Kit (gDNA Removal Column)
Tissue and Cell RNA Extraction Kit (gDNA Removal Column)

This product utilizes the latest genomic DNA removal column technology to ensure the effective removal of gDNA The resulting RNA does not require DNase digestion and can directly used for experiments


Product Introduction
introduction

Code No

Reagent

Price

NG3001S

50T

Request a quote

NG3001M 100T Request a quote
Introduction
This product utilizes the latest genomic DNA removal column technology to ensure the effective removal of gDNA The resulting RNA does not require DNase digestion and can directly used for experiments such as reverse transcription PCR and quantitative real-time PCR. A unique lysis buffer rapidly lyses cells and inactivates cellular RNases, after which the lysate is through a genomic DNA removal column, where genomic DNA is removed and RNA passes through the filter. After the filtered RNA is adjusted for binding conditions with ethanol, it is selectively adsorbed onto silica matrix membrane in the spin column under high-chaotropic salt conditions. Then, through a series of rapid wash-and-centrifugation steps, impurities such as cellular and proteins are removed. Finally, low-salt RNase-free water elutes the purified RNA from the silica matrix membrane.

Download: Manual (PDF)